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PoPS duplexer device

This device has two PoPS outputs which in terms of activity should be duplicates of the input.

PoPS duplexers are needed if we want to preserve the strict PoPS input/output interface of devices.

Implementation alternatives

General Duplexer Simplified Duplexer Duplexing by Concatenation
General PoPS Duplexer
Simplified Duplexer
Duplexing by Concatenation
PoPS Duplexer Variants

General Duplexer

The general duplexer variant offers most flexibility since both oututs can be controlled seperately. Ribosome binding sites of different strengths can be used to have different amplification factors on the ouptuts - which can be important if subsequent devices require input signals of different strengths. Another working point are the promoters, different promoter strengths also affect output signal strength. Note that some ribosome binding sites are missing in the illustration.

Simplified Duplexer

The simplified duplexer still facilitate specific output amplification, however, the only starting point is the promoters. Since both promoters are stimulated by the same activator, it might be more difficult to find appropriate promoters.

Duplexing by Concatenation

The direct way of PoPS duplexing is simple concatenation of two devices. The former device's output ends with the promoter - concatenation here means simply appending the inputs sequence (starting with ribosome binding site) of the subsequent device. Multiple input sequences of different gates can easily be appended. However, we loose the property of designated PoPS input/PoPS output boundaries for our devices.

For simplicity, we have anyway decided to use concatenation for PoPS duplexing - at least for the first experiments.


simulation results

Assembly procedure

Test procedure

Test results


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