Recombinational Enahancer: Part BBa J3101

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Recombinational Enhancer: Part BBa_J3101[1]

These are the oligos that we ordered to make the recombinational enhancer.

  • The underlined portion of T1 is an EcoRI sticky end
  • The underlined portion of T3 is a PstI sticky end site
  • The dark blue portions of the top strand oligoes are the bio brick ends.

5'-->3'

T1: AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT

T2: CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC

T3: ATTTTACTAGTTGCGGCCGCCTGCA

B1: GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA

B2: AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG


AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGCATTTTACTAGTTGCGGCCGCCTGCA

    GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGAAAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG

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