Template:ETH Sim Input Rate
From 2006.igem.org
The input rates (PoPS) have been chosen in the range of mRNA transcription rate, which was estimated according to the following assumptions:
- E.coli cytoplasm volume is approximately 6.7*10-16 l
- average number of mRNA molecules: 10
→ concentrationmRNA = 10/(6.7*10-16 * 6.022*1023) M = 0.0248 μM - at equilibrum, mRNA rate and degredation balance each other. Assuming half life period of 30min for mRNA, the result is
→ ratemRNA = concentrationmRNA * log(2)/30 μM/min = 5.7265e-04 μM/min
Amplifying/damping the input rates by small constant factors has influence on the qualitative outcome of the simulation.
- it is thus important to know how strong the input of the gate has to be.
- we can regulate this by choosing/designing the predecessor gate accordingly or
- by changing the ribosome binding sites to strengthen/weaken the input signal.
We accounted for this by adding restriction enzyme sites to the DNA, so that we can cut out current RBS to replace it by another of different strength.
