Imperial College 2006/Sandbox
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==Our Open Documentation== | ==Our Open Documentation== | ||
__NOTOC__ | __NOTOC__ | ||
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/LabCalendar <font face="century gothic" font style="color: #FFFFFF">Lab Notebook</font>] | *[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/LabCalendar <font face="century gothic" font style="color: #FFFFFF">Lab Notebook</font>] | ||
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Photos <font face="century gothic" font style="color: #FFFFFF">Photos</font>] | *[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Photos <font face="century gothic" font style="color: #FFFFFF">Photos</font>] | ||
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==The Team and Acknowledgements== | ==The Team and Acknowledgements== | ||
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- | ==Undergrads== | + | ==<font face="century gothic" font style="color: #FFFFFF">Undergrads</font>== |
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- | *[http://openwetware.org/wiki/User:Cys Christin Sander] | + | *[http://openwetware.org/wiki/User:Cys <font face="century gothic" font style="color: #FFFFFF">Christin Sander</font>] |
- | *[http://openwetware.org/wiki/User:da104 Deepti Aswani] | + | *[http://openwetware.org/wiki/User:da104 <font face="century gothic" font style="color: #FFFFFF">Deepti Aswani</font>] |
- | *[http://openwetware.org/wiki/User:Farah Farah Vohra] | + | *[http://openwetware.org/wiki/User:Farah <font face="century gothic" font style="color: #FFFFFF">Farah Vohra</font>] |
- | *[http://openwetware.org/wiki/User:Baijiongjun Jiongjun Bai] | + | *[http://openwetware.org/wiki/User:Baijiongjun<font face="century gothic" font style="color: #FFFFFF"> Jiongjun Bai</font>] |
- | *[http://openwetware.org/wiki/User:Johnsy John Sy] | + | *[http://openwetware.org/wiki/User:Johnsy <font face="century gothic" font style="color: #FFFFFF">John Sy</font>] |
- | *[http://openwetware.org/wiki/User:JohnChattaway John Chattaway] | + | *[http://openwetware.org/wiki/User:JohnChattaway <font face="century gothic" font style="color: #FFFFFF">John Chattaway</font>] |
- | *[http://openwetware.org/wiki/User:Jonny Jonathan Wells] | + | *[http://openwetware.org/wiki/User:Jonny<font face="century gothic" font style="color: #FFFFFF"> Jonathan Wells</font>] |
- | *[http://openwetware.org/wiki/User:Tom Tom Hinson] | + | *[http://openwetware.org/wiki/User:Tom <font face="century gothic" font style="color: #FFFFFF">Tom Hinson</font>] |
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[[Image:IGEM GroupPhotos0015.JPG|thumb|center|200px|The Imperial College iGEM team and advisors on the terrace of the Biochemistry building]] | [[Image:IGEM GroupPhotos0015.JPG|thumb|center|200px|The Imperial College iGEM team and advisors on the terrace of the Biochemistry building]] | ||
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<center> | <center> | ||
- | ==Advisors== | + | ==<font face="century gothic" font style="color: #FFFFFF">Advisors</font>== |
</center> | </center> | ||
- | *[http://www3.imperial.ac.uk/people/r.kitney Prof. Richard Kitney] | + | *[http://www3.imperial.ac.uk/people/r.kitney <font face="century gothic" font style="color: #FFFFFF">Prof. Richard Kitney</font>] |
- | *[http://www3.imperial.ac.uk/people/p.freemont Prof. Paul Freemont] | + | *[http://www3.imperial.ac.uk/people/p.freemont <font face="century gothic" font style="color: #FFFFFF">Prof. Paul Freemont</font>] |
- | *[http://www3.imperial.ac.uk/people/d.mann Dr. David Mann] | + | *[http://www3.imperial.ac.uk/people/d.mann <font face="century gothic" font style="color: #FFFFFF">Dr. David Mann</font>] |
- | *Kirsten Jensen | + | *<font face="century gothic" font style="color: #FFFFFF">Kirsten Jensen</font> |
- | *[http://openwetware.org/wiki/User:Vincent Vincent Rouilly] | + | *[http://openwetware.org/wiki/User:Vincent <font face="century gothic" font style="color: #FFFFFF">Vincent Rouilly</font>] |
- | *[http://openwetware.org/wiki/User:chuehloo Chueh-Loo Poh] | + | *[http://openwetware.org/wiki/User:chuehloo <font face="century gothic" font style="color: #FFFFFF">Chueh-Loo Poh</font>] |
- | *[http://openwetware.org/wiki/User:SxE00 Matthieu Bultelle] | + | *[http://openwetware.org/wiki/User:SxE00 <font face="century gothic" font style="color: #FFFFFF">Matthieu Bultelle</font>] |
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Revision as of 11:31, 30 October 2006
Oscillators are a fundamental building block in many engineering fields and are a widespread phenomenon in biology. Building a biological oscillator is thus a crucial step forward in the field of Synthetic Biology. | Following the design of the oscillator, a full theoretical analysis of the Lotka-Volterra properties was carried out, which promised the successful outcome of oscillations. Additionally, all components as well as the overall oscillator were modelled such that the behaviour of the system could be predicted. Our team successfully built functional parts, thus providing the building block for the final oscillator. All parts created were experimentally tested and their characterization could be used to feedback information into the modelling. |
Main Project
Secondary Projects
[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/popsblocker PoPs Blocker] | [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Bio_elec_interface Biological to Electrical Interface] |
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As a method of controlling the activation of the positive-feedback loop in our predator-prey based oscillator, we successfully created this part, which can be used as a general Pops Blocker:
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We also worked on a Biosensor for measuring AHL concentrations in order to establish a Biological to Electrical Interface this summer.
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Our Contributions to the Registry
Part Logo | Description | Link to registry | Built | Tested | Characterized | Sequenced | Sent |
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Final Constructs | |||||||
Final Prey Cell | J37015 | YES | YES | Pending | YES | YES | |
Cre/Lox Prey Control | J37027 | YES | YES | YES | YES | YES | |
Test Constructs | |||||||
Final Polycistronic Predator Cell Test Construct | J37016 | YES | YES | YES | YES | YES | |
Predator Cell, pLux Transfer Function (two promoters) | J37020 | YES | YES | NO | NO | YES | |
Intermediate Parts | |||||||
AHL induced LuxR generator (for predator cell) | J37019 | YES | N/A | N/A | YES | YES | |
RBS + LuxR | J37033 | YES | N/A | N/A | YES | YES | |
Prey Cell Intermediate | J37034 | YES | N/A | N/A | YES | YES | |
LuxR + GFP | J37032 | YES | N/A | N/A | YES | YES |
Our Open Documentation
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The Team and Acknowledgements
Undergrads
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Advisors
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Acknowledgements
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Funding
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