"E colock" Chiba 2006

(Difference between revisions)

Revision as of 06:35, 30 October 2006

『mu』team

Kai system

Kai system：the simplest and the most well known clock-system with a period of about 24h.

Kai system is driven by phosphorylation cascade composed of three proteins, such as KaiA,KaiB, and KaiC.

The oscillation of Kai system is converted to transcription cycle by SasA and RpaA.

This cycle is observable by produced fluorescent proteins.

08.06: １.Seek　for　an appropriate　promoter　and　fluoresceint　protein　for this project.←NOW!!
２.Obtain　KaiABC　and　SasA.

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 == Concept ==
-[[Image:CONCEPT.jpg|650px]]
+[[Image:CONCEPT.jpg|550px]]
 == How It Works ==
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 This cycle is observable by produced fluorescent proteins.
-[[
+[[Image:Kai system×E.jpg|450px]]
-== Techinical Issues ==
-'''1.Installing Kai Operon'''
-The DNA is installable, whether the Kai system in cyanobacteria is function in E.coli or not?
-'''2.Lack of Synchronization Mechanism'''
-Cyanobacteria use light for “reseting” the cycle, thus its population keeps synchronizing.
-However the genes for “reset bottom” has not been identified.
-→→①Placing Lac represser under KaiA and KaiBC promoter,Kai oscillation is all stopped.
-The population is synchronized.
-→→②Can we use AI??
-== Option ==
-'''Control of cycle duration'''
-There have been KaiA mutants with altered cycle periods.
-We want to do a similar to create “E clock” s with various cycle periods on demand.
 == Work Schedule and Progress ==
 ;08.06 :１.Seek　for　an appropriate　promoter　and　fluoresceint　protein　for this project.[[『mu』team activities|←NOW!!]]<br>２.Obtain　KaiABC　and　SasA.