Aggregation Behavior

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We have a great interest in some form of emergent phenomena and group dynamics based on simple local rules and external stimulae. Examples would be behaviors like sorting, pattern detections, flocking etc.
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The idea would be to have two types of E. coli :
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One type would express GFP (they look green)
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The other type would express RFP (they look red)
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The starting material is an unorganized mixture of red and green bacteria
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The GFP expressing bacteria would start, under induction, to express Antigen 43 (Ag43)which is an aggregation factor working on stable media culture.
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The "green" bacteria would start to gather making green dots on the plates.
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The "red" bacteria express, with a certain delay(long enough to let the green ones to cluster first), the aggregation factor (Ag43).  
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This would produce a sorted phenotype (green bacteria surrounded by red bacteria)

Latest revision as of 18:33, 29 July 2005

The idea would be to have two types of E. coli :

One type would express GFP (they look green)

The other type would express RFP (they look red)


The starting material is an unorganized mixture of red and green bacteria


The GFP expressing bacteria would start, under induction, to express Antigen 43 (Ag43)which is an aggregation factor working on stable media culture.


The "green" bacteria would start to gather making green dots on the plates.


The "red" bacteria express, with a certain delay(long enough to let the green ones to cluster first), the aggregation factor (Ag43).


This would produce a sorted phenotype (green bacteria surrounded by red bacteria)

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