Aggregation Behavior
From 2006.igem.org
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| - | The idea would be to have two types of E. coli | + | The idea would be to have two types of E. coli : |
One type would express GFP (they look green) | One type would express GFP (they look green) | ||
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The other type would express RFP (they look red) | The other type would express RFP (they look red) | ||
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| + | The starting material is an unorganized mixture of red and green bacteria | ||
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| + | The GFP expressing bacteria would start, under induction, to express Antigen 43 (Ag43)which is an aggregation factor working on stable media culture. | ||
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| + | The "green" bacteria would start to gather making green dots on the plates. | ||
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| + | The "red" bacteria express, with a certain delay(long enough to let the green ones to cluster first), the aggregation factor (Ag43). | ||
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| + | This would produce a sorted phenotype (green bacteria surrounded by red bacteria) | ||
Latest revision as of 18:33, 29 July 2005
The idea would be to have two types of E. coli :
One type would express GFP (they look green)
The other type would express RFP (they look red)
The starting material is an unorganized mixture of red and green bacteria
The GFP expressing bacteria would start, under induction, to express Antigen 43 (Ag43)which is an aggregation factor working on stable media culture.
The "green" bacteria would start to gather making green dots on the plates.
The "red" bacteria express, with a certain delay(long enough to let the green ones to cluster first), the aggregation factor (Ag43).
This would produce a sorted phenotype (green bacteria surrounded by red bacteria)
