Amplification Seeding
From 2006.igem.org
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[[McGill_University_2006|Home]] <br> | [[McGill_University_2006|Home]] <br> | ||
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+ | *1. Prepare desired volume of 1X LB + Antibiotic in culture tubes. | ||
+ | *2. Pipet 100-200uL of overnight culture into the fresh LB. | ||
+ | *3. Place in S-I until desired O.D. is reached. |
Revision as of 20:33, 12 July 2006
- 1. Prepare desired volume of 1X LB + Antibiotic in culture tubes.
- 2. Pipet 100-200uL of overnight culture into the fresh LB.
- 3. Place in S-I until desired O.D. is reached.