Amplification Seeding
From 2006.igem.org
(Difference between revisions)
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| - | 1. Prepare desired volume of 1X LB + Antibiotic in culture tubes. | + | 1. Prepare desired volume of 1X LB + Antibiotic in culture tubes. <br> |
| - | 2. Pipet 100-200uL of overnight culture into the fresh LB. | + | 2. Pipet 100-200uL of overnight culture into the fresh LB. <br> |
3. Place in S-I until desired O.D. is reached. | 3. Place in S-I until desired O.D. is reached. | ||
Revision as of 20:33, 12 July 2006
1. Prepare desired volume of 1X LB + Antibiotic in culture tubes.
2. Pipet 100-200uL of overnight culture into the fresh LB.
3. Place in S-I until desired O.D. is reached.
