Amplification Seeding
From 2006.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 2: | Line 2: | ||
[[Protocols|Protocols]] | [[Protocols|Protocols]] | ||
| - | + | 1. Prepare desired volume of 1X LB + Antibiotic in culture tubes. <br> | |
| - | 1. Prepare desired volume of 1X LB + Antibiotic in culture tubes. | + | 2. Pipet 100-200uL of overnight culture into the fresh LB. <br> |
| - | 2. Pipet 100-200uL of overnight culture into the fresh LB. | + | |
3. Place in S-I until desired O.D. is reached. | 3. Place in S-I until desired O.D. is reached. | ||
Latest revision as of 20:33, 12 July 2006
1. Prepare desired volume of 1X LB + Antibiotic in culture tubes.
2. Pipet 100-200uL of overnight culture into the fresh LB.
3. Place in S-I until desired O.D. is reached.
