Answer

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Depending on whether or not we want to be able to do multiple flips, we can use the Cre/LoxP to turn off (remove Hin), and use IPTG/LacR to turn on the Hin.
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We can use the Cre/LoxP to turn off (remove Hin), and use IPTG/LacR (we also want an inducer that is able to go away such as ''Lactose'') to turn on the Hin.
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Being able to turn the Hin on "''and''" off would be more beneficial for future use, so we feel that we should try and do both.

Latest revision as of 14:37, 30 May 2006

We can use the Cre/LoxP to turn off (remove Hin), and use IPTG/LacR (we also want an inducer that is able to go away such as Lactose) to turn on the Hin. Being able to turn the Hin on "and" off would be more beneficial for future use, so we feel that we should try and do both.

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