Answer T1

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
 
(2 intermediate revisions not shown)
Line 1: Line 1:
-
For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange.   
+
For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange.   
-
Unclear whether these conditions could be established in E coli.
+
 
 +
Unclear whether these conditions could be established in E coli.
 +
 
 +
In [http://www.kip.uni-heidelberg.de/chromcon/publications/pdf-files/Rippe_NAR_97.pdf Rippe et al. 1997], superhelical density of a 1868 bp plasmid was -0.03, with 80% of plasmids having -6 supercoils.

Latest revision as of 16:32, 30 May 2006

For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange.

Unclear whether these conditions could be established in E coli.

In [http://www.kip.uni-heidelberg.de/chromcon/publications/pdf-files/Rippe_NAR_97.pdf Rippe et al. 1997], superhelical density of a 1868 bp plasmid was -0.03, with 80% of plasmids having -6 supercoils.

Personal tools
Past/present/future years