Answers to this one and the next
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R.C. Johnson and M.F. Bruist. 1989. Intermediates in Hin-mediated DNA inversion: a role for Fis and the recombinational enhancer in the strand exchange reaction | R.C. Johnson and M.F. Bruist. 1989. Intermediates in Hin-mediated DNA inversion: a role for Fis and the recombinational enhancer in the strand exchange reaction | ||
[http://www.pubmedcentral.gov/picrender.fcgi?tool=pmcentrez&blobtype=pdf&artid=400990] | [http://www.pubmedcentral.gov/picrender.fcgi?tool=pmcentrez&blobtype=pdf&artid=400990] | ||
- | + | #RE sequence must be present | |
- | + | #w/o RE, Hin can still assemble to with the two Hix’s however, Hin can’t generate inversion | |
- | + | #Prevention of Fis binding causes loss of enhancer function | |
- | + | #60 bp in length | |
- | + | #Binds two Fis proteins (98 AA’s) | |
- | + | #Enhancer cannot function when located too close to a recombination site | |
- | + | #HU facilitates the required bending of the DNA to create the inversion interaction | |
- | + | #HU stimulation is dependent upon location of RE | |
- | + | #Dependence upon HU is lesser when distance between RE and recombination site is greater | |
- | + | #Required to induce the bending of the shorter, yet stiffer, segment of DNA | |
- | + | #When RE is located >650 bp for the recombination site the dependence upon HU to form the complex is lost | |
- | + | #RE combined w/ supercoiling mediate the rotation of DNA strands subsequent to double-stranded cleavage by Hin within two recombination sites | |
Haykinson and Johnson. 1993. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly | Haykinson and Johnson. 1993. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly | ||
[http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8508775] | [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8508775] | ||
- | + | #The two recombination sites bound by Hin are assembled together at the Fis-bound RE | |
- | + | #w/o HU invertasome assembly is very inefficient | |
- | + | #“HU is the only protein in E. coli that can promote invertasome formation with | |
short DNA lengths between RE and recombination sites | short DNA lengths between RE and recombination sites | ||
- | + | #HU binds non-specifically to DNA between the enhancer and recombination site to facilitate DNA looping | |
- | + | #“The RE can function at great distances !!! >4 kb !!! and on either side of a recombination site on a plasmid to stimulate Hin-mediated DNA inversion” | |
- | + | #RE is not functional at a position 32 bp minimum | |
- | + | #Due to restricted looping |
Revision as of 16:31, 30 May 2006
Recombinase Enhancer R.C. Johnson and M.F. Bruist. 1989. Intermediates in Hin-mediated DNA inversion: a role for Fis and the recombinational enhancer in the strand exchange reaction [http://www.pubmedcentral.gov/picrender.fcgi?tool=pmcentrez&blobtype=pdf&artid=400990]
- RE sequence must be present
- w/o RE, Hin can still assemble to with the two Hix’s however, Hin can’t generate inversion
- Prevention of Fis binding causes loss of enhancer function
- 60 bp in length
#Binds two Fis proteins (98 AA’s)
- Enhancer cannot function when located too close to a recombination site
- HU facilitates the required bending of the DNA to create the inversion interaction
- HU stimulation is dependent upon location of RE
- Dependence upon HU is lesser when distance between RE and recombination site is greater
#Required to induce the bending of the shorter, yet stiffer, segment of DNA
- When RE is located >650 bp for the recombination site the dependence upon HU to form the complex is lost
- RE combined w/ supercoiling mediate the rotation of DNA strands subsequent to double-stranded cleavage by Hin within two recombination sites
Haykinson and Johnson. 1993. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8508775]
- The two recombination sites bound by Hin are assembled together at the Fis-bound RE
- w/o HU invertasome assembly is very inefficient
- “HU is the only protein in E. coli that can promote invertasome formation with
short DNA lengths between RE and recombination sites
- HU binds non-specifically to DNA between the enhancer and recombination site to facilitate DNA looping
- “The RE can function at great distances !!! >4 kb !!! and on either side of a recombination site on a plasmid to stimulate Hin-mediated DNA inversion”
- RE is not functional at a position 32 bp minimum
#Due to restricted looping