BU06:Research
From 2006.igem.org
Making E. coli Glow
Preliminary Design: -Light Sensing Inhibitor- -> -Light emission device-
Light Sensing Inihibitor: BBa_I15008 , BBa_I15009 , BBa_I15010 , BBa_R0082
Light Emission Device: (from lux operon) THIS IS WHAT WE NEED TO DO.
Light Sensor Parts: http://partsregistry.org/Featured_Parts:Light_Sensor and paper : http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html (this paper simply explains how the device works!)
It seems to repress gene expression by having red light inhibit phosphorylation which would activate a promoter. We would replace the LacZ protein coding region with our lux operon.
Sequence details:
According to Tom Knight, the Photorhabdus luminescens luxCDABE operon that he cloned is NCBI accession number M90093. I checked this sequence against the biobrick restriction enzymes (EcoRI, XbaI, SpeI, PstI, NotI) using the Sequence Manipulation Suite 2
