BU06:Team A

(Difference between revisions)

Revision as of 21:38, 10 July 2006

Schedule	Research	Fundraising	Resources	Members	Contact

Team Goal: Photography System Assembly and Testing

Mac Cowell	macowell@bu.edu	aim: cxmac
Christene Woods	cwoods@bu.edu	aim: xoptopinope
Nancy Mendonca Davis	nancymd@bu.edu
Nadav Ivzan	nivzan@bu.edu	aim:kingos82 msn:kingos19@hotmail.com

=== Protocols ===

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 == Current Progress ==
-===Making ''E. coli'' Glow===
+===Photosystem parts===
-'''Preliminary Design: ''-Light Sensing Inhibitor- -> -Light emission device-'' '''
 ''Light Sensing Inihibitor:'' [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] , [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] , [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] , [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]
-''Light Emission Device:'' (from lux [http://en.wikipedia.org/wiki/Operon operon]) THIS IS WHAT WE NEED TO DO. [[Lux_operon| Lux Operon]]
-Point Mutation: Lamda Red - Datsenko, Wanner... or by PCR: look for protocol on OpenWetWare\
-Codon Usage Bias Database: http://www.kazusa.or.jp/codon/
-Light Sensor Parts: http://partsregistry.org/Featured_Parts:Light_Sensor and paper : http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html (this paper simply explains how the device works!)
-It seems to repress gene expression by having red light inhibit phosphorylation which would activate a promoter. We would replace the LacZ protein coding region with our lux [http://en.wikipedia.org/wiki/Operon operon].
-Part: BBa_F1610 codes for LuxI, should we need it...
+===Assembly of parts===