BU06:Team A
From 2006.igem.org
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== References == | == References == | ||
| + | * [http://openwetware.org/wiki/Synthetic_Biology:BioBricks Synthetic_Biology:BioBricks] TONS of overlooked info & protocols on OWW. | ||
* The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates. | * The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates. | ||
* The [http://partsregistry.org/Featured_Parts:Light_Sensor Light Sensing System]. | * The [http://partsregistry.org/Featured_Parts:Light_Sensor Light Sensing System]. | ||
Revision as of 22:53, 13 July 2006
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| Schedule | Research | Fundraising | Resources | Members | Contact |
|---|
| Team Goal: Photography System Assembly and Testing |
back to the BU iGEM main page
Contents |
Members
| Mac Cowell | macowell@bu.edu | aim: cxmac2 | |
| Christine Woods | cwoods@bu.edu | aim: xoptopinope | |
| Nancy Mendonca Davis | nancymd@bu.edu | ||
| Nadav Ivzan | nivzan@bu.edu | aim:kingos82 msn:kingos19@hotmail.com |
Meeting Schedule
Next meeting: Thursday, July 13th 4pm LSEB Basement
Current Progress
Photosystem parts
Light Sensing Inhibitor:
- BBa_I15008 (well: 21A plate: iGEM2006 DNA-2)
- BBa_I15009 (well: 21C plate: iGEM2006 DNA-2)
- BBa_I15010 (Note: Must be used in E.coli deficient in natural EnvZ. Also, not in 2006 iGEM dna distribution)
- BBa_R0082 (well: 15P plate: iGEM2006 DNA-1)
Assembly of parts
- Biobrick delivery - overview of the the 2006 iGEM DNA distribution plates.
- Color-codes that indicate the corresponding biobrick's antibiotic resistance. (Each brick has a certain antibiotic resistance. Biobricks stored in Plate 1 are all Ampicilin resistant. Those in Plate 2 have a color-code).
- biobrick construction tutoral and related protocols.
- Summary of biobrick construction from the Silver lab.
- 3A assembly is an interesting alternative that avoids the gel purification step.
Questions
- What strain of e. coli should we use? How do we make those cells competent?
- How do we find out which antibiotic a particular DNA on Plate 2 is resistant to? The Biobrick Delivery instructions say that we should use the Registry Repository to find out, but it doens't seem to list each part's antibiotic resistance on there.
- The bottom of each well is color-coded to indicate the antibiotic resistance.
- How do we obtain part I15010?
- Email Meagan at the registry (meaganl AT mit DOT edu) and copy James Brown (jrb62 AT cam.ac.uk)
- How do we obtain EnvZ- e. coli?
- Ask frank - he seems to know.
- Who knows what we can buy now and what general lab supplies are available for our use?
Protocols
References
- Synthetic_Biology:BioBricks TONS of overlooked info & protocols on OWW.
- The index of the 384-well plates.
- The Light Sensing System.
- Protocols
- Reference Part M30109
- Bacterial Photography Lab w/M30109 part.
