BU06:Team A
From 2006.igem.org
|
| Schedule | Research | Fundraising | Resources | Members | Contact |
|---|
| Team Goal: Photography System Assembly and Testing |
back to the BU iGEM main page
Contents |
Members
| Mac Cowell | macowell@bu.edu | aim: cxmac2 | |
| Christine Woods | cwoods@bu.edu | aim: xoptopinope | |
| Nancy Mendonca Davis | nancymd@bu.edu | ||
| Nadav Ivzan | nivzan@bu.edu | aim:kingos82 msn:kingos19@hotmail.com |
Meeting Schedule
Next meeting: Wednesday, July 12th After general meeting
Current Progress
Photosystem parts
Light Sensing Inhibitor:
- BBa_I15008 (well: 21A plate: iGEM2006 DNA-2)
- BBa_I15009 (well: 21C plate: iGEM2006 DNA-2)
- BBa_I15010 (Note: Must be used in E.coli deficient in natural EnvZ.)
- BBa_R0082 (well: 15P plate: iGEM2006 DNA-1)
Assembly of parts
Here are instructions for transforming DNA into cells and creating our own stock of DNA from the 2006 iGEM plates as well as figuring out where your part is on the plates.
Note: Any DNA in the DNA 1 plate is Ampicillin resistant but the DNA 2 plate contains either Ampicillin, Ampicillin-Kanamycin, and Kanamycin resistant DNA...we're still figuring out how to determine each part's resistance.
Questions
- What strain of e. coli should we use? How do we make those cells competent?
- How do we find out which antibiotic a particular DNA on Plate 2 is resistant to? The Biobrick Delivery instructions say that we should use the Registry Repository to find out, but it doens't seem to list each part's antibiotic resistance on there.
- The bottom of each well is color-coded to indicate the antibiotic resistance.
- How do we obtain part I15010?
- Email Meagan at the registry (meaganl AT mit DOT edu) and copy James Brown (jrb62 AT cam.ac.uk)
- How do we obtain EnvZ- e. coli?
- Ask frank - he seems to know.
- Who knows what we can buy now and what general lab supplies are available for our use?
Protocols
References
- The index of the 384-well plates.
- The Light Sensing System.
