|Team Goal: Photography System Assembly and Testing|
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|Mac Cowellfirstname.lastname@example.org||aim: cxmac2|
|Christine Woodsemail@example.com||aim: xoptopinope|
|Nancy Mendonca Davisfirstname.lastname@example.org|
|Nadav Ivzanemail@example.com||aim:kingos82 msn:firstname.lastname@example.org|
Light Sensing Inhibitor:
- BBa_I15008 (well: 21A plate: iGEM2006 DNA-2)
- BBa_I15009 (well: 21C plate: iGEM2006 DNA-2)
- BBa_I15010 (Note: Must be used in E.coli deficient in natural EnvZ. Also, not in 2006 iGEM dna distribution)
- BBa_R0082 (well: 15P plate: iGEM2006 DNA-1)
Assembly of parts
- Biobrick delivery - overview of the the 2006 iGEM DNA distribution plates.
- Color-codes that indicate the corresponding biobrick's antibiotic resistance. (Each brick has a certain antibiotic resistance. Biobricks stored in Plate 1 are all Ampicilin resistant. Those in Plate 2 have a color-code).
- biobrick construction tutoral and related protocols.
- What strain of e. coli should we use? How do we make those cells competent?
- How do we find out which antibiotic a particular DNA on Plate 2 is resistant to? The Biobrick Delivery instructions say that we should use the Registry Repository to find out, but it doens't seem to list each part's antibiotic resistance on there.
- The bottom of each well is color-coded to indicate the antibiotic resistance.
- How do we obtain part I15010?
- Email Meagan at the registry (meaganl AT mit DOT edu) and copy James Brown (jrb62 AT cam.ac.uk)
- How do we obtain EnvZ- e. coli?
- Ask frank - he seems to know.
- Who knows what we can buy now and what general lab supplies are available for our use?
- Synthetic_Biology:BioBricks TONS of overlooked info & protocols on OWW.
- The index of the 384-well plates.
- The Light Sensing System.
- Reference Part M30109
- Bacterial Photography Lab w/M30109 part.
- Background on the lux operon
- Bioluminescence. Interesting.