BU06:Team C

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=== Techniques ===
=== Techniques ===
<br><br>
<br><br>
-
[http://www.openwetware.org/wiki/Designing_primers Designing Primers]
+
*[http://www.openwetware.org/wiki/Designing_primers Designing Primers]
=== Protocols ===
=== Protocols ===
-
[http://www.openwetware.org/wiki/Agarose_gel_electrophoresis Agarose Gel Electrophoresis]
+
*[http://www.openwetware.org/wiki/Agarose_gel_electrophoresis Agarose Gel Electrophoresis]
-
[http://www.openwetware.org/wiki/DNA_Ligation DNA Ligation]
+
*[http://www.openwetware.org/wiki/DNA_Ligation DNA Ligation]
-
[http://www.openwetware.org/wiki/Miniprep/Qiagen_kit Miniprep]
+
*[http://www.openwetware.org/wiki/Miniprep/Qiagen_kit Miniprep]
-
[http://www.openwetware.org/wiki/Restriction_digest Restriction Digest]
+
*[http://www.openwetware.org/wiki/Restriction_digest Restriction Digest]
=== Tools ===
=== Tools ===
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[http://tools.neb.com/NEBcutter2/index.php NEB Cutter]
+
*[http://tools.neb.com/NEBcutter2/index.php NEB Cutter]
-
[http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ IDT Oligo Analyzer]
+
*[http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ IDT Oligo Analyzer]

Revision as of 16:11, 6 July 2006

BU06.jpg
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Team Goal: Add Restriction Sites to Biobrick Ends

back to the BU iGEM main page


Contents

Meeting Schedule

Wednesday July 12th, 3pm Ingalls

  • Goal: Present info on how to isolate from plasmid, how to PCR and add ends, and protocol/primer design


Current Progress

  • Adding Ends PCR! We will use the AgeI restriction site in the middle where two PCR reactions will overlap and we can stick em together!
  • How can we test if the restriction sites have been added successfully?

References

Techniques



  • [http://www.openwetware.org/wiki/Designing_primers Designing Primers]

Protocols

  • [http://www.openwetware.org/wiki/Agarose_gel_electrophoresis Agarose Gel Electrophoresis]
  • [http://www.openwetware.org/wiki/DNA_Ligation DNA Ligation]
  • [http://www.openwetware.org/wiki/Miniprep/Qiagen_kit Miniprep]
  • [http://www.openwetware.org/wiki/Restriction_digest Restriction Digest]

Tools

  • [http://tools.neb.com/NEBcutter2/index.php NEB Cutter]
  • [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ IDT Oligo Analyzer]

Personal tools
Past/present/future years