BU06:Team D
From 2006.igem.org
(Difference between revisions)
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== Current Progress == | == Current Progress == | ||
| + | ===Silent Point Mutation: XbaI at bp 2411 of P. luminescens LuxCDABE=== | ||
| + | _/_____ <---XbaI cut | ||
| + | 5' AAT CTA GAT 3' | ||
| + | N L D | ||
| + | Asn Leu Asp | ||
| + | |||
| + | Triplets for these A.A.'s | ||
| + | N L D | ||
| + | AAT TTA GAT | ||
| + | AAC TTG GAC | ||
| + | CTG | ||
| + | CTT | ||
| + | CTC | ||
| + | CTA | ||
| + | |||
| + | Codon Usage Bias! (Frequency: per thousand) | ||
| + | Leu Codon E.coliK12 P.lumin | ||
| + | CUU 11.0 12.1 | ||
| + | CUC 11.0 9.1 | ||
| + | CUA 3.9 9.0 | ||
| + | CUG 52.8 29.6 | ||
| + | CUA in P.lum is 9.0/1000, for a silent point mutation of Leucine, CUU or CUC with frequency of 11.0/1000... | ||
| + | and its still Leu!!!! | ||
Revision as of 19:04, 26 June 2006
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Contents |
Meeting Schedule
Current Progress
Silent Point Mutation: XbaI at bp 2411 of P. luminescens LuxCDABE
_/_____ <---XbaI cut
5' AAT CTA GAT 3'
N L D
Asn Leu Asp
Triplets for these A.A.'s
N L D
AAT TTA GAT
AAC TTG GAC
CTG
CTT
CTC
CTA
Codon Usage Bias! (Frequency: per thousand)
Leu Codon E.coliK12 P.lumin
CUU 11.0 12.1
CUC 11.0 9.1
CUA 3.9 9.0
CUG 52.8 29.6
CUA in P.lum is 9.0/1000, for a silent point mutation of Leucine, CUU or CUC with frequency of 11.0/1000... and its still Leu!!!!
References
Techniques
