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These books are online at ncbi (searchable, but not directly browseable):
- Tons of protocols at OpenWetWare
- Invitrogen iProtocols
- Quigen's Handbooks & protocols - be sure to check out the Bench Guide under "General Molecular biology protocols"
- Current Protocols in Molecular Biology from Wiley InterScience
- Promega Guides
- Misc. links at the main iGEM Resources page (used to be "teaching resources" page
- standard assembly
- Abstraction hierarchy and PoPS
- Standard E. coli Strain for BioBricks
- Synthetic Biology articles in DSpace:
- BE.109: RT-PCR data analysis
- VecScreen has a list of all the vector nucleotide sequences in ncbi.
- VectorDB contains annotations and sequence information for many vectors (2600+) commonly used in molecular biology.
- PlasMapper generates sweet annotated vector-based plasmid maps from a web interface.
- Why didn't the Registry include empty resistance plasmids in the DNA shipment?
- Check the Registry FAQ.
- Standard Assembly
- the basics of building and manipulating Parts, including details about XbaI and SpeI, the restriction enzymes that are the basis of these operations.
- RBS-CDS design issues
- how the ribosomal binding site is reconciled with the location of the BioBrick ends.
- Key to our design
- Lux operon in the Registry Glossary
- LuxCDABE listing in prodoric.
- Virtual Footprint at prodoric searches for prokaryote promoters in a given sequence.
- An overview of UT Austin's Light Sensor system.
Team members can store and reference PDFs of scientific papers at http://www.citeulike.org/user/BUiGEM/ (Get the password from Richard) Take a look at tagging feature and the bookmarklet used for posting. (I'll put in more details soon). Here's a list of tags I have so far:
- part2b for papers describing DNA sequences that we could turn into a part
- part_XYZ for papers describing a specific part we might make
- gene_XYZ for papers describing a particular gene in any detail
Remember you can apply any number of relevant tags you like to a given paper.
Tom Knight's earlier plasmid construction technique: http://web.mit.edu/synbio/release/docs/biobricks.pdf
Randy Rettberg sez B0015 is a pretty reliable terminator.