Balloon Bacteria : Chiba 2006

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
Line 2: Line 2:
[[Image:Balloonconcept.jpg]]
[[Image:Balloonconcept.jpg]]
-
When light shines on E. coli,,,
+
The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.
-
'''E. coli become bigger and bigger like BALLOON !!!'''  
+
Suprise!!
 +
 
 +
The E. coli become bigger and bigger like BALLOON!!!  
 +
 
 +
'''→World first bacteria that swells by lights.!!!'''
 +
 
 +
 
 +
=Design=
 +
 
 +
to stop the e.coli by light, we needed a part that stop the e.coli, and combine this with a light receptor that UCSF made it last year.
 +
 
 +
there was some ways to stop the e.coli running:
 +
#making a chemaera with chemotaxis receptor and light sensing receptor
 +
#stop the motor protein's expression(motB protein: from last year's parts)
 +
#controling chemotaxis
 +
etc
 +
 +
we chose to control chemotaxis.
 +
We've found out, from the paper(..see refrences), the chemoreceptor that fix the e.coli tumble.
 +
it is a fragment of the chemoreceptor "Tsr" (a serin receptor)from aa 290 to 551. we call this tsr-cw(or tsr290).
 +
 
 +
<br />
 +
[[Image:pathway.jpg|pathway]]

Revision as of 07:49, 30 October 2006

Concept

Balloonconcept.jpg

The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.

Suprise!!

The E. coli become bigger and bigger like BALLOON!!!

→World first bacteria that swells by lights.!!!


Design

to stop the e.coli by light, we needed a part that stop the e.coli, and combine this with a light receptor that UCSF made it last year.

there was some ways to stop the e.coli running:

  1. making a chemaera with chemotaxis receptor and light sensing receptor
  2. stop the motor protein's expression(motB protein: from last year's parts)
  3. controling chemotaxis

etc

we chose to control chemotaxis. We've found out, from the paper(..see refrences), the chemoreceptor that fix the e.coli tumble. it is a fragment of the chemoreceptor "Tsr" (a serin receptor)from aa 290 to 551. we call this tsr-cw(or tsr290).


pathway


Member

Chiba 2006 [iGEM Chiba]

『je』team

Personal tools
Past/present/future years