Berkeley2006-NetworksMain

From 2006.igem.org

Revision as of 04:27, 28 October 2006 by JCAnderson (Talk | contribs)
Jump to: navigation, search

Nodes are Built from Multiple Cell Types

Berkeley2006 Net2.GIF

Concentration in Culture Gives Graded Responses

Berkeley2006 Net3.GIF

Trainable Networks are Built from Nodes

Berkeley2006 Net1.GIF


Each cell has a complete lock-dependent communication system

Each cell has a unique lock address and a key message to send

Communication between a sender and a receiver is therefore restricted to cells that are a donor with the key that unlocks the recipient's lock address

There are many lock and key pairs, but the partnering between potential communicating cells is restricted in layers--layer 1 cells only contain keys that correspond to layer 2 locks. Layer 2 keys only unlock layer 3 locks, etc.

Within a layer, each n+1 layer lock component has a key with all permutations of layer n. If each layer has 10 addresses (locks) , there are 10x10, or 100 unique genotypes within each member of a layer is able to communicate with each member of the previous layer.

At the top of the layers is a set of training cells which direct a positive selectable marker to cells that contain their key sequence. The 'input' to the training system is therefore a set of key sequences.

At the end of the layers is a set of training cells that direct a negatively selectable marker, or "kill" signal contained in their key sequence. All cells able to receive the kill signal die.


Next Section: Synthetic Promoters

Personal tools
Past/present/future years