Berkeley Work Schedule and Progress
From 2006.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | == <Current Work: == | |
| - | + | Conjugation testing:<br> | |
| - | + | - oriT - Test plasmid mobility<br> | |
| - | == <Current Work: | + | - TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> |
| - | Conjugation testing: | + | - Test mobilization construct<br> |
| + | Construction:<br> | ||
| + | - Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | ||
| + | Other | ||
- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | ||
| - | + | ||
Revision as of 08:46, 14 November 2005
<Current Work:
Conjugation testing:
- oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
Construction:
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
Other
- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
