Berkeley Work Schedule and Progress
From 2006.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
- TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> | - TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> | ||
- Test mobilization construct<br> | - Test mobilization construct<br> | ||
| - | Construction:<br> | + | ''Construction:''<br> |
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | - Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | ||
Other | Other | ||
Revision as of 08:47, 14 November 2005
<Current Work:
Conjugation testing:
- oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
Construction:
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
Other
- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
