Berkeley Work Schedule and Progress
From 2006.igem.org
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== Current Work: == | == Current Work: == | ||
| - | ''< | + | ''<red>Conjugation testing:</red>''<br> |
- oriT - Test plasmid mobility<br> | - oriT - Test plasmid mobility<br> | ||
- TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> | - TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> | ||
Revision as of 08:49, 14 November 2005
Current Work:
<red>Conjugation testing:</red>
- oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
Construction:
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
Other
- Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones:
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
