Berkeley Work Schedule and Progress

From 2006.igem.org

Current Work:

<r>Conjugation testing:</r>
- oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
Construction:
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf Other - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)

Milestones:

November:
Measurement data for constructs, construction continues

September:
Project training continues, implementation begins

July-August:
Theoretical project design and background research on bacterial conjugation and technique training

May-June:
Synthetic Biology primer and project brainstorming