Berkeley Work Schedule and Progress

From 2006.igem.org

Current Work:

1. Conjugation testing:
   
   1. - oriT - Test plasmid mobility
      
   2. - TraJf, TraJr - does promoter induction cause conjugation to initiate?
      
   3. - Test mobilization construct
      
2. Construction:
   
   1. - Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
3. Other
   1. - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)

Milestones:

November:
Measurement data for constructs, construction continues

September:
Project training continues, implementation begins

July-August:
Theoretical project design and background research on bacterial conjugation and technique training

May-June:
Synthetic Biology primer and project brainstorming