Berkeley Work Schedule and Progress

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Revision as of 09:03, 14 November 2005 by 24.7.103.65 (Talk)
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Current Work:

  • Conjugation testing:
    • oriT - Test plasmid mobility
    • TraJf, TraJr - does promoter induction cause conjugation to initiate?
    • Test mobilization construct
  • Construction:
    • Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
  • Lock and Key Strength
    • Flourescence assays
  • Other
    • onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)


Milestones:

November:
Measurement data for constructs, construction continues

September:
Project training continues, implementation begins

July-August:
Theoretical project design and background research on bacterial conjugation and technique training

May-June:
Synthetic Biology primer and project brainstorming

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