Biobrick delivery


Revision as of 19:13, 20 April 2006 by Meaganl (Talk | contribs)
Jump to: navigation, search

One of the resources that MIT provides teams competing in iGEM is a copy of parts that have been submitted to the Registry. These parts also include those that were submitted as a result of previous iGEM competitions (a big reason why we want you to keep on top of sending us your parts as you create them!). For iGEM 2006, the Registry will send out parts in the form of dry DNA.

Sometime in the next several weeks you will receive a package in the mail containing a plate that looks like this:

Top of plate containing dry DNA
Bottom of plate containing dry DNA

What do you do with this plate once you have received it?

  • The DNA at the bottom of the wells needs to be resuspended. To do this you must:
  1. Peel off the foil cover OR puncture a whole through the foil with a pipette tip
  2. Add X ul of TE Buffer (Tris-HCl?)
  3. Take 1ul DNA and transform into your favorite competent cells, plate out on a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.
  4. Pick a single colony and inoculate some broth (with the correct antibiotic) and grow ~18 hours.
  5. Use the resulting culture to miniprep AND make your own glycerol stock (for further instruction on making a glycerol see this page).

This DNA is your own collection of parts that you can use to create your own parts. By making your own glycerols you now have your own stock to go back to and grow/prep whenever you need that particular part.

What is that red (or orange, green, yellow) stuff at the bottom of the wells?

Personal tools
Past/present/future years