Boston University 2006:making antibiotic stocks

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(in progress - off to go verify and test it.)
(→Method: added the table with lovely web-safe colors)
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==Method==
==Method==
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Table of concentrations goes here
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{| border="1" cellpadding="4" style="text-align:center"
 +
|+ Common antibiotic stock & working concentrations
 +
|-
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!width="120"|Antibiotic
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!width="50"|Abrv.
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!width="75"|Working
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!width="75"|Stock
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!Notes
 +
!Color Code
 +
|- style="background:#ff9900;"
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| Ampicillin || Amp || 100 ug/mL || 100 mg/mL || || orange
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|- style="background:#66ff66;"
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| Chloramphenicol || Chm || 170 ug/mL || 170 mg/mL || in 100% ethanol || green
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|- style="background:#ff6666;"
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| Kanamycin || Kan || 50 ug/mL || 50 mg/mL || || red
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|- style="background:#ffff66;"
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| Tetracycline HCL || Tet || 50 ug/mL || 50 mg/mL || in 100% ethanol || yellow
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|}
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 +
 
# a
# a

Revision as of 19:54, 7 September 2006

Use the following protocol to produce 5 1mL aliquots of stock solution of your desired antibiotic. Use a 1:1000 dilution to reach a working concentration from the stock (i.e. take 5uL from the Amp stock and add to 5mL of LB broth to produce 100ug/mL Amp + LB broth).

Materials

  • Powder of given antibiotic
  • dH20 and/or 100% ethanol
  • 5mL Falcon tube(s)
  • 5 1.5mL microcentrifuge tubes (per 5mL falcon tube)

Method

Common antibiotic stock & working concentrations
Antibiotic Abrv. Working Stock Notes Color Code
Ampicillin Amp 100 ug/mL 100 mg/mL orange
Chloramphenicol Chm 170 ug/mL 170 mg/mL in 100% ethanol green
Kanamycin Kan 50 ug/mL 50 mg/mL red
Tetracycline HCL Tet 50 ug/mL 50 mg/mL in 100% ethanol yellow


  1. a
  2. b

Notes

  • Store all the aliquots at -20C.
  • Tet is light-sensative, so be sure to prevent exposure (i.e. wrap it in tinfoil or keep it in a dark box)
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