Brown:Equipment needed

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| 1
| 1
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| cell shaker/incubator| | 1|-  
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| cell shaker/incubator
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| source of ddH2O| | 1|-  
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|  
-
| hot plates w/ stirring| | 2|-  
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| 1
-
| stir bars (set)| | 1|-  
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|-  
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| PCR thermocycler| | 1|-  
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| source of ddH2O
-
| microcentrifuge (w/temp cntrl.)| | 1|-  
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|  
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| centrifuge | 50mL and 15mL| 1|-  
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| 1
-
| Electrophoresis gel set| | 4|-  
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|-  
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| Gel Power source| | 1|-  
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| hot plates w/ stirring
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| UV Gel viewer| | 1|-  
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|  
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| UV Gel camera| | 1|-  
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| 2
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| Pipettemen| | |-  
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|-  
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| | 20μL| 4|-  
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| stir bars (set)
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| | 200μL| 4|-  
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|  
-
| | 1000μL| 4|-  
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| 1
-
| Electronic pipettor| can use 1-25mL pipettes| 1|-  
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|-  
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| bunsen burners| | 2|-  
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| PCR thermocycler
 +
|  
 +
| 1
 +
|-  
 +
| microcentrifuge (w/temp cntrl.)
 +
|  
 +
| 1
 +
|-  
 +
| centrifuge  
 +
| 50mL and 15mL
 +
| 1
 +
|-  
 +
| Electrophoresis gel set
 +
|  
 +
| 4
 +
|-  
 +
| Gel Power source
 +
|  
 +
| 1
 +
|-  
 +
| UV Gel viewer
 +
|  
 +
| 1
 +
|-  
 +
| UV Gel camera
 +
|  
 +
| 1
 +
|-  
 +
| Pipettemen
 +
|  
 +
|  
 +
|-  
 +
|  
 +
| 20μL
 +
| 4
 +
|-  
 +
|  
 +
| 200μL
 +
| 4
 +
|-  
 +
|  
 +
| 1000μL
 +
| 4
 +
|-  
 +
| Electronic pipettor
 +
| can use 1-25mL pipettes
 +
| 1
 +
|-  
 +
| bunsen burner
 +
|  
 +
| 2
 +
|-  
| sparkers| | 2|-  
| sparkers| | 2|-  
| wire loop| to pick a bac clone| 3|-  
| wire loop| to pick a bac clone| 3|-  

Revision as of 22:27, 17 May 2006

Equipment type/size quantity Status
freezers/fridges
-80°C 1
-20°C 1
4°C 1
incubator 1
hot bath 1
cold bath 1
autoclave 1
cell shaker/incubator 1
source of ddH2O 1
hot plates w/ stirring 2
stir bars (set) 1
PCR thermocycler 1
microcentrifuge (w/temp cntrl.) 1
centrifuge 50mL and 15mL 1
Electrophoresis gel set 4
Gel Power source 1
UV Gel viewer 1
UV Gel camera 1
Pipettemen
20μL 4
200μL 4
1000μL 4
Electronic pipettor can use 1-25mL pipettes 1
bunsen burner 2
| 2|- to pick a bac clone| 3|- to spread bacteria| 3|- | |- | |- type/size| quantity|- | |- 1L| 4|- 500mL| 8|- 100mL| 8|- | |- 1L| 2|- 250mL| 8|- | |- 50mL| 100|- 15mL| 100|- | |- 500mL| 2|- 100mL| 2|- | |- 1mL| 2|- 5mL| 2|- 10mL| 2|- 25mL| 2|- | |- small| 1|- medium| 4|- large| 4|- | |- | |- | |- | |- 1.5mL| 2000|- 200μL (PCR)| 1000|- | |- 20μL| a lot|- 200μL| a lot|- 1000μL| a lot|- | 200|- | |- (polypropylene)| 100|- | |- for 1.5mL tubes| 4|- 2|- Reagents 70% ethanol 
      $ 400.00   Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>   custom primers   
      $ 800.00   Antibodies for identifying cell division proteins 4 x $200   
      $ 250.00   disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)   
      $ 600.00   Gel kit: Agarose gel aparatus, power source   
         TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros   
         Taq polymerase kit, DNTP - Free bases (actg ready mixed)   
      $ 600.00   Restriction enzymes  $60 x 10   
      $ 300.00   Thermocycler   
      $ 500.00   misc. equipment and supplies   
      $ 4,000.00   DNA synthesis costs $1.40 per base.   
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
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