Brown:Summer workshops

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*molar vs. normal solutions
*molar vs. normal solutions
*buffers
*buffers
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 +
*HOMEWORK!!!
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**1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. 
'''Led by:''' Ana (10am-12pm)
'''Led by:''' Ana (10am-12pm)

Revision as of 21:24, 19 May 2006

Monday June 5, 2006


Lab Safety

  • clothing/protection
  • common sense
  • waste
  • storage

Overview of research basics

  • lab notebook
  • resources
  • why am I doing this experiment?

Making stock solutions

  • pipetting
  • weighing
  • graduated cylinders
  • volumetric flasks
  • % solutions (vol/vol, g/vol)
  • molar vs. normal solutions
  • buffers
  • HOMEWORK!!!
    • 1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid].

Led by: Ana (10am-12pm)
Lab safety training with engineering group from 1pm - 5pm Please email Karen to join group. All students must have completed lab safety training to work in the lab.

Tuesday June 6, 2006


Restriction digest
Gel electrophoresis
Led by: Azeem and Jason

Wednesday June 7, 2006


Primer Design
PCR/Gel
Led by: Azeem and Jason

Thursday June 8, 2006


Bacterial cell culture
Bacterial transfection
Making plates
Led by: Jamie Gagnon


Friday June 9, 2006


Mini-prep
Led by: Azeem and Jason

Personal tools
Past/present/future years