http://2006.igem.org/wiki/index.php?title=Brown:Transformation_Protocol_with_Fischer_cells&feed=atom&action=history
Brown:Transformation Protocol with Fischer cells - Revision history
2024-03-29T06:47:26Z
Revision history for this page on the wiki
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http://2006.igem.org/wiki/index.php?title=Brown:Transformation_Protocol_with_Fischer_cells&diff=12209&oldid=prev
Jamielemon at 19:27, 6 July 2006
2006-07-06T19:27:55Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:27, 6 July 2006</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. INCUBATE ON ICE FOR 30 MIN.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. INCUBATE ON ICE FOR 30 MIN.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>5. HEAT SHOCK AT 42˚C FOR 30 SEC.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>5. HEAT SHOCK AT 42˚C <ins class="diffchange diffchange-inline">IN WATER BATH </ins>FOR 30 SEC.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>6. PLACE ON ICE FOR 2 MIN.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>6. PLACE ON ICE FOR 2 MIN.</div></td></tr>
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Jamielemon
http://2006.igem.org/wiki/index.php?title=Brown:Transformation_Protocol_with_Fischer_cells&diff=12198&oldid=prev
Agao at 19:09, 6 July 2006
2006-07-06T19:09:50Z
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<p><b>New page</b></p><div>[[Brown:Lab Protocols |Back]] <br><br><br />
TRANSFORMATION PROTOCOL WITH FISCHER COMPETENT CELLS<br />
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1. TAKE CELLS FROM -80 AND THAW FOR IMMEDIATE USE<br />
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2. MIX CELLS GENTLY WITH TAPPING<br />
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3. ADD 4µL DNA TO 50µL COMPETENT CELLS (EACH VIAL CONTAINS 50µL)<br />
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4. INCUBATE ON ICE FOR 30 MIN.<br />
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5. HEAT SHOCK AT 42˚C FOR 30 SEC.<br />
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6. PLACE ON ICE FOR 2 MIN.<br />
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7. ADD 250µL CIRCLE GROW<br />
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8. INCUBATE FOR 1 HOUR WITH SHAKING AT 37˚C<br />
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9. ADD 250µL CIRLCE GROW<br />
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10. PLATE 200µL OF MIX</div>
Agao