Construction
From 2006.igem.org
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| Line 19: | Line 19: | ||
<li>Miniprep Q04400 (2005) (2006) and Q04510 (2005) (2006) | <li>Miniprep Q04400 (2005) (2006) and Q04510 (2005) (2006) | ||
<li>Replate UT1 with miniprep from a good conlony | <li>Replate UT1 with miniprep from a good conlony | ||
| - | <li> | + | <li>Miniprep Q04400 (2005) (2006) Q04510 (2005) (2006) and check to see if bands are correct. If |
| + | so, digest with EcoRI/XbaI and gel extract | ||
| + | <li>Ligate with UT1 | ||
</ul> | </ul> | ||
Revision as of 05:32, 19 October 2006
< Aug11-Sep7 | Sep8-14 | Sep15-26 | Sep27-Oct3 | Oct4-Oct13 | Construction >
October 18, 2006
Andy, Konstantin:
- Transformed and plated Q03400 (2005) (2006)
- Gel-extracted UT1 DEF with EcoRI/SpeI (2500-3000, 5000, 8000), 8000 band bright, digestion appeared incomplete
- Made A, K, AK plates
- Made o/n of Q04400 (2005) (2006) Q04510 (2005) (2006)
- Tested UT3 in DH5a with 0%-2% arabinose
To-Do List:
- Make o/n of Q03400 (2005) (2006)
- Miniprep Q04400 (2005) (2006) and Q04510 (2005) (2006)
- Replate UT1 with miniprep from a good conlony
- Miniprep Q04400 (2005) (2006) Q04510 (2005) (2006) and check to see if bands are correct. If so, digest with EcoRI/XbaI and gel extract
- Ligate with UT1
October 14, 2006
Charles, Jovan:
- Mini-prepped UT2 2/4 and UT3 7
- Transform UT2 2/4 and UT3 7 into DH5a-z1 and DH5a cells
- Continue with the IPTG test for the rest of the colonies that we weren’t able to test yesterday
To-Do List:
- Measure absorbance spectrum of Arabinose
- Make o/n of working UT2/UT3 for repeat of IPTG test.
- Look into using tetR and tet pL instead of cI and Prm+
