Construction Intermediates

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Line 1: Line 1:
'''pBad Promoter- RBS''' BBa_J3102 [http://partsregistry.org/Part:BBa_J3102]
'''pBad Promoter- RBS''' BBa_J3102 [http://partsregistry.org/Part:BBa_J3102]
 +
* Used culture #3
'''pBad Promoter-Hix''' : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529]
'''pBad Promoter-Hix''' : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529]
 +
*Used the Midi-prepped DNA that we recieved from the Mammoths
 +
 +
'''pLac Promoter-RBS''' :
'''pBad Promotor-Hix-RBS''' : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530]
'''pBad Promotor-Hix-RBS''' : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530]
 +
* Used culture #3
'''Recombinational Enhancer- Double Forward terminator'''- BBa_S03512 [http://partsregistry.org/Part:BBa_S03512]
'''Recombinational Enhancer- Double Forward terminator'''- BBa_S03512 [http://partsregistry.org/Part:BBa_S03512]
Line 10: Line 15:
'''Double Forward Terminator- Recombinational Enhancer''': BBa_S03516 [http://partsregistry.org/Part:BBa_S03516]
'''Double Forward Terminator- Recombinational Enhancer''': BBa_S03516 [http://partsregistry.org/Part:BBa_S03516]
 +
*Used culture #1
'''HinLVA-Double Forward Terminator''': BBa_S03514 [http://partsregistry.org/Part:BBa_S03514]
'''HinLVA-Double Forward Terminator''': BBa_S03514 [http://partsregistry.org/Part:BBa_S03514]
 +
* Used culture #1
'''Hin-Double Forward Terminator''':  BBa_S03515 [http://partsregistry.org/Part:BBa_S03515]
'''Hin-Double Forward Terminator''':  BBa_S03515 [http://partsregistry.org/Part:BBa_S03515]
 +
* Used culutre #3
'''Hix-Double Forward Terminator-RE''' : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522]
'''Hix-Double Forward Terminator-RE''' : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522]
 +
*Used culture #1
'''TetR Forwards-HixC''': BBa_S03523 [http://partsregistry.org/Part:BBa_S03523]
'''TetR Forwards-HixC''': BBa_S03523 [http://partsregistry.org/Part:BBa_S03523]
 +
* Used culutre #2
'''TetR Forwards-Hix- Double Forward Terminator-RE''': BBa_S03524 [http://partsregistry.org/Part:BBa_S03524]
'''TetR Forwards-Hix- Double Forward Terminator-RE''': BBa_S03524 [http://partsregistry.org/Part:BBa_S03524]
 +
* Used culture #2
'''Hix-TetR Forwards-Hix-Double Forward Terminator-RE''': BBa_S03527 [http://partsregistry.org/Part:BBa_S03527]
'''Hix-TetR Forwards-Hix-Double Forward Terminator-RE''': BBa_S03527 [http://partsregistry.org/Part:BBa_S03527]
 +
* Used culture # 2
'''TetR Backwards-Hix''' : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525]
'''TetR Backwards-Hix''' : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525]
 +
* Used culture #4
'''TetR Backwards-Hix- Double Forward Terminator-RE''': BBa_S03526 [http://partsregistry.org/Part:BBa_S03526]
'''TetR Backwards-Hix- Double Forward Terminator-RE''': BBa_S03526 [http://partsregistry.org/Part:BBa_S03526]
 +
* Used culture # 3
'''Hix-TetR Backwards-Hix-Double Forward Terminator-RE''': BBa_S03528 [http://partsregistry.org/Part:BBa_S03528]
'''Hix-TetR Backwards-Hix-Double Forward Terminator-RE''': BBa_S03528 [http://partsregistry.org/Part:BBa_S03528]
 +
*Used culture #3

Revision as of 15:16, 2 August 2006

pBad Promoter- RBS BBa_J3102 [http://partsregistry.org/Part:BBa_J3102]

  • Used culture #3

pBad Promoter-Hix : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529]

  • Used the Midi-prepped DNA that we recieved from the Mammoths

pLac Promoter-RBS :

pBad Promotor-Hix-RBS : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530]

  • Used culture #3

Recombinational Enhancer- Double Forward terminator- BBa_S03512 [http://partsregistry.org/Part:BBa_S03512]

  • This part has a twin, S03513, that was made accidentally
  • Both parts were created on the registry by accident. It was never made in the laboratory.

Double Forward Terminator- Recombinational Enhancer: BBa_S03516 [http://partsregistry.org/Part:BBa_S03516]

  • Used culture #1

HinLVA-Double Forward Terminator: BBa_S03514 [http://partsregistry.org/Part:BBa_S03514]

  • Used culture #1

Hin-Double Forward Terminator: BBa_S03515 [http://partsregistry.org/Part:BBa_S03515]

  • Used culutre #3

Hix-Double Forward Terminator-RE : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522]

  • Used culture #1

TetR Forwards-HixC: BBa_S03523 [http://partsregistry.org/Part:BBa_S03523]

  • Used culutre #2

TetR Forwards-Hix- Double Forward Terminator-RE: BBa_S03524 [http://partsregistry.org/Part:BBa_S03524]

  • Used culture #2

Hix-TetR Forwards-Hix-Double Forward Terminator-RE: BBa_S03527 [http://partsregistry.org/Part:BBa_S03527]

  • Used culture # 2

TetR Backwards-Hix : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525]

  • Used culture #4

TetR Backwards-Hix- Double Forward Terminator-RE: BBa_S03526 [http://partsregistry.org/Part:BBa_S03526]

  • Used culture # 3

Hix-TetR Backwards-Hix-Double Forward Terminator-RE: BBa_S03528 [http://partsregistry.org/Part:BBa_S03528]

  • Used culture #3
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