Construction Intermediates
From 2006.igem.org
Nicknames for the parts are in blue
pBad Promoter- RBS BBa_J3102 [1]
- Used culture #3
pBad Promoter-Hix : BBa_S03529 [2]
- Used the Midi-prepped DNA that we recieved from the Mammoths
pLac Promoter-RBS : BBa_S03511 [3]
- Used culture #4
pBad Promotor-Hix-RBS (P-H-R) : BBa_S03530 [4]
- Used culture #3
Recombinational Enhancer- Double Forward terminator(RE-TT): BBa_S03512 [5]
- This part has a twin, S03513, that was made accidentally
- Both parts were created on the registry by accident. It was never made in the laboratory.
Double Forward Terminator- Recombinational Enhancer (TT-RE) : BBa_S03516 [6]
- Used culture #1
HinLVA-Double Forward Terminator(HLVA-TT): BBa_S03514 [7]
- Used culture #1
Hin-Double Forward Terminator(H-TT): BBa_S03515 [8]
- Used culutre #3
Hix-Double Forward Terminator-RE(H-TT-RE) : BBa_S03522 [9]
- Used culture #1
TetR Forwards-HixC(TF-H): BBa_S03523 [10]
- Used culutre #2
TetR Forwards-Hix- Double Forward Terminator-RE(TF-H-TT-RE): BBa_S03524 [11]
- Used culture #2
Hix-TetR Forwards-Hix-Double Forward Terminator-RE(H-TF-H-TT-RE) : BBa_S03527 [12]
- Used culture # 2
TetR Backwards-Hix(TB-H) : BBa_S03525 [13]
- Used culture #4
TetR Backwards-Hix- Double Forward Terminator-RE(TB-H-TT-RE) : BBa_S03526 [14]
- Used culture # 3
Hix-TetR Backwards-Hix-Double Forward Terminator-RE(H-TB-H-TT-RE): BBa_S03528 [15]
- Used culture #3
TetR Backwards-RBS(backwards)(TB-RBSrev) : BBa_S03532 [16]
- Used culture #2
pBad-Hix C- TetR Backwards-RBS (backwards)(P-H-TB-RBSrev) : BBa_S03533 [17]
- Used culture #1
TetR Backwards- RBS Backwards-HixC- Double Forwards Terminator-RE(TB-RBSrev-H-TT-RE) BBa_S03534 [18]
- We chose not to use this part for ligation.
Double Terminator Forwards-RBS-TetR Forwards(TTfwd-RBS-TF) BBa_S03595 [19]
- Constructed in plasmid pSB1AK3, then put into plasmid pSB1A2. Used culture #'s 1, 2 and 3 (combined)