Construction Intermediates

From 2006.igem.org

Nicknames for the parts are in blue

pBad Promoter- RBS BBa_J3102 [1]

• Used culture #3

pBad Promoter-Hix : BBa_S03529 [2]

• Used the Midi-prepped DNA that we recieved from the Mammoths

pLac Promoter-RBS : BBa_S03511 [3]

• Used culture #4

pBad Promotor-Hix-RBS (P-H-R) : BBa_S03530 [4]

• Used culture #3

Recombinational Enhancer- Double Forward terminator(RE-TT): BBa_S03512 [5]

• This part has a twin, S03513, that was made accidentally
• Both parts were created on the registry by accident. It was never made in the laboratory.

Double Forward Terminator- Recombinational Enhancer (TT-RE) : BBa_S03516 [6]

• Used culture #1

HinLVA-Double Forward Terminator(HLVA-TT): BBa_S03514 [7]

• Used culture #1

Hin-Double Forward Terminator(H-TT): BBa_S03515 [8]

• Used culutre #3

Hix-Double Forward Terminator-RE(H-TT-RE) : BBa_S03522 [9]

• Used culture #1

TetR Forwards-HixC(TF-H): BBa_S03523 [10]

• Used culutre #2

TetR Forwards-Hix- Double Forward Terminator-RE(TF-H-TT-RE): BBa_S03524 [11]

• Used culture #2

Hix-TetR Forwards-Hix-Double Forward Terminator-RE(H-TF-H-TT-RE) : BBa_S03527 [12]

• Used culture # 2

TetR Backwards-Hix(TB-H) : BBa_S03525 [13]

• Used culture #4

TetR Backwards-Hix- Double Forward Terminator-RE(TB-H-TT-RE) : BBa_S03526 [14]

• Used culture # 3

Hix-TetR Backwards-Hix-Double Forward Terminator-RE(H-TB-H-TT-RE): BBa_S03528 [15]

• Used culture #3

TetR Backwards-RBS(backwards)(TB-RBS_rev) : BBa_S03532 [16]

• Used culture #2

pBad-Hix C- TetR Backwards-RBS (backwards)(P-H-TB-RBS_rev)  : BBa_S03533 [17]

• Used culture #1

TetR Backwards- RBS Backwards-HixC- Double Forwards Terminator-RE(TB-RBS_rev-H-TT-RE) BBa_S03534 [18]

• We chose not to use this part for ligation.

Double Terminator Forwards-RBS-TetR Forwards(TT_fwd-RBS-TF) BBa_S03595 [19]

• Constructed in plasmid pSB1AK3, then put into plasmid pSB1A2. Used culture #'s 1, 2 and 3 (combined)