Davidson 2006
From 2006.igem.org
Contents |
Students
• Sabriya Rosemond [1] is a rising junior biology major at Hampton University in VA.
• Erin Zwack [2] is a rising junior biology major at Davidson College in NC.
• Lance Harden [3] is a rising sophomore at Davidson College, who might major in math.
Faculty
• A. Malcolm Campbell Department of Biology, [4]
• Laurie J. Heyer Department of Mathematics, [5]
Papers of Interest
• The Effects of Ethidium Bromide and Mg++ Ion on Strand Exchange in the Hin-mediated Inversion Reaction. Hee Jung Lee et al. Excellent description of Hin recombination mechanism.
• The Effects of Symmetrical Recombination Site hixC on Hin Recombinase Function. Heon Man Lim et al.
• The role of the loxP spacer region in P1 site-specific recombination. Ronald H.Hoess et al.
• Non-contact positions impose site selectivity on Cre recombinase. Andreas W. Rufer and Brian Sauer
• Growth Phase-Dependent Variation in Protein Composition of the Escherichia coli Nucleoid
• Hin-mediated Inversion on Positively Supercoiled DNA
Project Description
Parts Created
Questions
Assembly Issues
Current Course of Action
Plasmids
- pSB1A3
- amp resistant
- copy number:100-300
- size:2157 empty
- pSB1AC3
- part-BBAp1000
- copy number:100-3000
- amp and CmR resistant
- contains device that provides CmR
- size:3844 ( with device) 3005 (without device)
- pSB1AK3
- part BBa_|13535
- copy number:100-300
- amp and kan resistance
- contains a device for screening test
- size: 3457 (with device) 3189 (without device)
- Transform bacteria We are using Salmonella typhimurium for the Hin protein as it was the referenced species in all of the literature.
- Create RE biobrick (completed)
- Isolating genomic DNA from Salmonella typhimurium
- Ordered olioges for recombinational enhancer and primers for the hin gene