ETH2006 and
From 2006.igem.org
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Part to be used in the prototype system: [http://partsregistry.org/Part:BBa_J34100 BBa_J34100] | Part to be used in the prototype system: [http://partsregistry.org/Part:BBa_J34100 BBa_J34100] | ||
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| + | An AND gate produces an output only if both input signals are present. We want to implement such a “device” by using a suppressor tRNA. In our proposed model Signal 1 would lead to the transcription of the T7 RNA polymerase. However, an early stop codon was introduced into the coding sequence, leading to the expression of a truncated, non-functional protein. Signal 2, on the other hand, will lead to the expression of a suppressor tRNA. This tRNA recognizes the stop codon, preventing the end of translation and leading to incorporation of a glutamine into the nascent amino acid chain, so that translation can be completed. Consequently, a functional T7 RNA polymerase is present which will recognize its cognate promoter (T7 promoter) and therefore initiate expression of the downstream reporter gene (mCherry). | ||
[[Image:ETH and trna parts.png| |center]] | [[Image:ETH and trna parts.png| |center]] | ||
Revision as of 09:40, 29 October 2006
Contents |
AND gate
The AND gate's PoPs output activity should be correlated to the PoPs input activity as shown in the picture:
input A ^
| L D H
| D D D
| L D L
+--------->
input B
output: High, Low, Dont care
implementation alternatives
Part to be used in the prototype system: BBa_J34100
An AND gate produces an output only if both input signals are present. We want to implement such a “device” by using a suppressor tRNA. In our proposed model Signal 1 would lead to the transcription of the T7 RNA polymerase. However, an early stop codon was introduced into the coding sequence, leading to the expression of a truncated, non-functional protein. Signal 2, on the other hand, will lead to the expression of a suppressor tRNA. This tRNA recognizes the stop codon, preventing the end of translation and leading to incorporation of a glutamine into the nascent amino acid chain, so that translation can be completed. Consequently, a functional T7 RNA polymerase is present which will recognize its cognate promoter (T7 promoter) and therefore initiate expression of the downstream reporter gene (mCherry).
modeling
AND-tRNA → simulation results / sensitivity analysis
