ETH2006 iptg
From 2006.igem.org
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[[Image:ETH_IPTG_sensing_parts.png|center|600px|complete system based on alternative 1]] | [[Image:ETH_IPTG_sensing_parts.png|center|600px|complete system based on alternative 1]] | ||
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+ | === modeling === | ||
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+ | [[ETH_Sim_Mod_IPTG|simulation results]] | ||
=== assembly procedure === | === assembly procedure === |
Revision as of 13:49, 26 October 2006
Contents |
chemical sensing device
The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment.
implementation alternatives
- Lactate lacI represses, IPTG induces (BBa_R0011 or BBa_R0010 )
- Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) (BBa_R0040)
- combination thereof (BBa_I13614 / BBa_13617 / BBa_I13623 / BBa_I13624 / BBa_I13627 / BBa_I13637 / BBa_I13653)
- simple sugar Arabinose (BBa_R0080)
I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. since the inducers are water-soluble we would have to fix the chemicals onto the petri dish.
modeling
assembly procedure
where we got it and link to theyer documentation
Progress:
- 2006/10/03
- Made LB-Agar plates with antibiotics
- 2006/10/04
- Transformed cells, plated them
- 2006/10/05
- Found plates empty after 18h on the table, put into incubator at 37°C
- 2006/10/06
- picked cultures onto fresh plates