ETH2006 iptg
From 2006.igem.org
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| - | == | + | == Chemical sensing device == |
The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment. | The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment. | ||
| - | === | + | === Implementation alternatives === |
# Lactate lacI represses, IPTG induces ([http://partsregistry.org/Part:BBa_R0011 BBa_R0011] or [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] ) | # Lactate lacI represses, IPTG induces ([http://partsregistry.org/Part:BBa_R0011 BBa_R0011] or [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] ) | ||
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# simple sugar Arabinose ([http://partsregistry.org/Part:BBa_R0080 BBa_R0080]) | # simple sugar Arabinose ([http://partsregistry.org/Part:BBa_R0080 BBa_R0080]) | ||
| - | I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. | + | I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. Since the inducers are water-soluble we would have to fix the chemicals onto the petri dish. |
[[Image:ETH_IPTG_sensing_parts.png|center|600px|complete system based on alternative 1]] | [[Image:ETH_IPTG_sensing_parts.png|center|600px|complete system based on alternative 1]] | ||
| - | === | + | === Modeling === |
[[ETH_Sim_Mod_IPTG|simulation results]] | [[ETH_Sim_Mod_IPTG|simulation results]] | ||
| - | === | + | === Assembly procedure === |
| - | + | ''Where we got it and link to theyer documentation'' | |
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Progress: | Progress: | ||
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;2006/10/06: picked cultures onto fresh plates | ;2006/10/06: picked cultures onto fresh plates | ||
| - | + | ''Marko J. continued from here'' | |
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| + | === Test procedure === | ||
| + | === Test results === | ||
| + | === Parts === | ||
→[[ETH Zurich 2006#system modeling|back to main page]] | →[[ETH Zurich 2006#system modeling|back to main page]] | ||
Revision as of 14:20, 29 October 2006
Contents |
Chemical sensing device
The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment.
Implementation alternatives
- Lactate lacI represses, IPTG induces (BBa_R0011 or BBa_R0010 )
- Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) (BBa_R0040)
- combination thereof (BBa_I13614 / BBa_13617 / BBa_I13623 / BBa_I13624 / BBa_I13627 / BBa_I13637 / BBa_I13653)
- simple sugar Arabinose (BBa_R0080)
I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. Since the inducers are water-soluble we would have to fix the chemicals onto the petri dish.
Modeling
Assembly procedure
Where we got it and link to theyer documentation
Progress:
- 2006/10/03
- Made LB-Agar plates with antibiotics
- 2006/10/04
- Transformed cells, plated them
- 2006/10/05
- Found plates empty after 18h on the table, put into incubator at 37°C
- 2006/10/06
- picked cultures onto fresh plates
Marko J. continued from here
