ETH2006 iptg

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
(chemical sensing device)
m
Line 1: Line 1:
-
== chemical sensing device ==
+
== Chemical sensing device ==
The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment.
The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment.
-
=== implementation alternatives ===
+
=== Implementation alternatives ===
# Lactate  lacI represses, IPTG induces ([http://partsregistry.org/Part:BBa_R0011 BBa_R0011] or [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] )  
# Lactate  lacI represses, IPTG induces ([http://partsregistry.org/Part:BBa_R0011 BBa_R0011] or [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] )  
Line 10: Line 10:
# simple sugar Arabinose ([http://partsregistry.org/Part:BBa_R0080 BBa_R0080])
# simple sugar Arabinose ([http://partsregistry.org/Part:BBa_R0080 BBa_R0080])
-
I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. since the inducers are water-soluble we would have to fix the chemicals onto the petri dish.
+
I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. Since the inducers are water-soluble we would have to fix the chemicals onto the petri dish.
[[Image:ETH_IPTG_sensing_parts.png|center|600px|complete system based on alternative 1]]
[[Image:ETH_IPTG_sensing_parts.png|center|600px|complete system based on alternative 1]]
-
=== modeling ===
+
=== Modeling ===
[[ETH_Sim_Mod_IPTG|simulation results]]
[[ETH_Sim_Mod_IPTG|simulation results]]
-
=== assembly procedure ===
+
=== Assembly procedure ===
-
 
+
''Where we got it and link to theyer documentation''
-
where we got it and link to theyer documentation
+
Progress:
Progress:
Line 29: Line 28:
;2006/10/06: picked cultures onto fresh plates
;2006/10/06: picked cultures onto fresh plates
-
=== test procedure ===
+
''Marko J. continued from here''
-
 
+
-
=== test results ===
+
-
 
+
-
=== parts ===
+
-
 
+
 +
=== Test procedure ===
 +
=== Test results ===
 +
=== Parts ===
→[[ETH Zurich 2006#system modeling|back to main page]]
→[[ETH Zurich 2006#system modeling|back to main page]]

Revision as of 14:20, 29 October 2006

Contents

Chemical sensing device

The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment.

Implementation alternatives

  1. Lactate lacI represses, IPTG induces ([http://partsregistry.org/Part:BBa_R0011 BBa_R0011] or [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] )
  2. Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040])
  3. combination thereof ([http://partsregistry.org/Part:BBa_I13614 BBa_I13614] / [http://partsregistry.org/Part:BBa_I13617 BBa_13617] / [http://partsregistry.org/Part:BBa_I13623 BBa_I13623] / [http://partsregistry.org/Part:BBa_I13624 BBa_I13624] / [http://partsregistry.org/Part:BBa_I13627 BBa_I13627] / [http://partsregistry.org/Part:BBa_I13637 BBa_I13637] / [http://partsregistry.org/Part:BBa_I13653 BBa_I13653])
  4. simple sugar Arabinose ([http://partsregistry.org/Part:BBa_R0080 BBa_R0080])

I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. Since the inducers are water-soluble we would have to fix the chemicals onto the petri dish.

complete system based on alternative 1

Modeling

simulation results

Assembly procedure

Where we got it and link to theyer documentation

Progress:

2006/10/03
Made LB-Agar plates with antibiotics
2006/10/04
Transformed cells, plated them
2006/10/05
Found plates empty after 18h on the table, put into incubator at 37°C
2006/10/06
picked cultures onto fresh plates

Marko J. continued from here

Test procedure

Test results

Parts

back to main page

Personal tools
Past/present/future years