ETH2006 iptg
From 2006.igem.org
Contents |
Chemical sensing device
The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment.
Implementation alternatives
- Lactate lacI represses, IPTG induces (BBa_R0011 or BBa_R0010 )
- Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) (BBa_R0040)
- combination thereof (BBa_I13614 / BBa_13617 / BBa_I13623 / BBa_I13624 / BBa_I13627 / BBa_I13637 / BBa_I13653)
- simple sugar Arabinose (BBa_R0080)
I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. Since the inducers are water-soluble we would have to fix the chemicals onto the petri dish.
Current implementation
We decided to use the LacI system with IPTG as chemical to be sensed.
Modeling
Assembly procedure
Where we got it and link to theyer documentation
Progress:
- 2006/10/03
- Made LB-Agar plates with antibiotics
- 2006/10/04
- Transformed cells, plated them
- 2006/10/05
- Found plates empty after 18h on the table, put into incubator at 37°C
- 2006/10/06
- picked cultures onto fresh plates
Marko J. continued from here