ETH2006 iptg

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Contents

Chemical sensing device

The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment.

Implementation alternatives

  1. Lactate lacI represses, IPTG induces (BBa_R0011 or BBa_R0010 )
  2. Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) (BBa_R0040)
  3. combination thereof (BBa_I13614 / BBa_13617 / BBa_I13623 / BBa_I13624 / BBa_I13627 / BBa_I13637 / BBa_I13653)
  4. simple sugar Arabinose (BBa_R0080)

I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. Since the inducers are water-soluble we would have to fix the chemicals onto the petri dish.

Current implementation

Since most parts were already existing in the registry, we decided to use the LacI system with IPTG as chemical to be sensed.

complete system based on alternative 1

Modeling

simulation results

Assembly procedure

Where we got it and link to theyer documentation

Progress:

2006/10/03
Made LB-Agar plates with antibiotics
2006/10/04
Transformed cells, plated them
2006/10/05
Found plates empty after 18h on the table, put into incubator at 37°C
2006/10/06
picked cultures onto fresh plates

Marko J. continued from here

Test procedure

Test results

Parts

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