Freiburg University 2006
From 2006.igem.org
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====Abstract==== | ====Abstract==== | ||
| - | The idea is to design a strand of DNA such that it wraps into some meaningful shape. There are three different stages for this project: First, the DNA should fold into a | + | The idea is to design a strand of DNA such that it wraps into some meaningful shape. There are three different stages for this project: First, the DNA should fold into a two-dimensional rectangular sheet. Secondly, this sheet should wrap itself up into the shape of a short pipe. And last, these little pipes should hook themselves up to each other such that they form one single long pipe. |
| - | ====Scetches==== | + | ====Scetches - Pipes ==== |
| - | + | [[image:Pipes1.jpg|left|thumb|200px|Pipes - Stage 1]] | |
| + | [[image:Pipes2.jpg|left|thumb|150px|Pipes - Stage 2]] | ||
| + | [[image:Pipes3.jpg|left|thumb|150px|Pipes - Stage 3]] | ||
| + | <br style="clear:both;"/> | ||
| + | |||
| + | The DNA sequence for the Pipes design can be found [http://omnibus.uni-freiburg.de/~kouznet/Tube-M13mp18-NEB-d.txt here]. | ||
===Application: Barbie Nanoatelier=== | ===Application: Barbie Nanoatelier=== | ||
Once the process of DNA folding into 3D-structures is understood, shapes can be chosen arbitrarily. The idea of the Barbie Nanoatelier is that the DNA should wrap into a 3D-TShirt, 3D-Pants, etc. | Once the process of DNA folding into 3D-structures is understood, shapes can be chosen arbitrarily. The idea of the Barbie Nanoatelier is that the DNA should wrap into a 3D-TShirt, 3D-Pants, etc. | ||
| + | |||
| + | ====Scetches - TShirt ==== | ||
| + | |||
| + | [[image:TShirt1.jpg|left|thumb|150px|TShirt - Stage 1]] | ||
| + | [[image:TShirt2.jpg|left|thumb|150px|TShirt - Stage 2]] | ||
| + | [[image:TShirt3.JPG|left|thumb|150px|TShirt - Stage 3]] | ||
| + | [[image:TShirt4.JPG|left|thumb|150px|TShirt - Stage 4]] | ||
| + | <br style="clear:both;"/> | ||
| + | |||
| + | ====Scetches - Bluse ==== | ||
| + | |||
| + | [[image:Bluse1.jpg|left|thumb|150px|Bluse - Stage 1]] | ||
| + | [[image:Bluse2.jpg|left|thumb|150px|Bluse - Stage 2]] | ||
| + | <br style="clear:both;"/> | ||
| + | |||
| + | The DNA sequence for the Bluse design can be found [http://omnibus.uni-freiburg.de/~kouznet/nike-oligos.txt here]. | ||
== GEM Freiburg == | == GEM Freiburg == | ||
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* [http://omnibus.uni-freiburg.de/~kouznet/GS-design.pdf Genetic systems design from the DNA modules] | * [http://omnibus.uni-freiburg.de/~kouznet/GS-design.pdf Genetic systems design from the DNA modules] | ||
| - | == | + | == Old project idea== |
* [http://omnibus.uni-freiburg.de/~kouznet/life6000.pdf Life with a price of 6000 €] | * [http://omnibus.uni-freiburg.de/~kouznet/life6000.pdf Life with a price of 6000 €] | ||
Revision as of 21:00, 28 August 2006
This term was invented by Martin Schneider on the Rule 110 Winter Workshop in 2004 [1]. We play without rules. We discover the rules that govern life, the universe and everything to exploit these rules and to create Artificial Life. Our short-time aim is the trip to Boston in November 2006 to take a prize in the iGEM.
The Freiburg Team (well, at least half of it...) from left to right: Irina Petrova, Andrei Kouznetsov, Daniel Hautzinger.
Contents |
The Team
Students:
iGEM instructor:
Faculty/staff:
The project: DNA Folding
Basic Idea:
Abstract
The idea is to design a strand of DNA such that it wraps into some meaningful shape. There are three different stages for this project: First, the DNA should fold into a two-dimensional rectangular sheet. Secondly, this sheet should wrap itself up into the shape of a short pipe. And last, these little pipes should hook themselves up to each other such that they form one single long pipe.
Scetches - Pipes
The DNA sequence for the Pipes design can be found here.
Application: Barbie Nanoatelier
Once the process of DNA folding into 3D-structures is understood, shapes can be chosen arbitrarily. The idea of the Barbie Nanoatelier is that the DNA should wrap into a 3D-TShirt, 3D-Pants, etc.
Scetches - TShirt
Scetches - Bluse
The DNA sequence for the Bluse design can be found here.
GEM Freiburg
Club
SB Preliminary
- DNA plug-and-play platform 24.05.06 27.06.06
- Very Large-Scale Integration design in Biology
- Genetic systems design from the DNA modules
Old project idea
Now we see it could be cheaper, but it will need a great intellectual impact. So, we’re opened for sponsors and relationships.
Comments
As I understand it, the idea is to create a "life automaton" consisting of 6 proteins? That is what I took out of your proposal. That seems quite ambitioned, but I can't really judge it, and even if it is quite ambitioned, that does not mean that it is bad, of course.
My understanding says that you are using 6000 € for denovo synthesis of 6 genes required for making minimal life. Right?
So, what if after all the simulations and tests on the computer, you order the DNA for minimal life and it turns out that it doesn't work... What will you do then?
This is because biology is unpredictable and you must not assume that the DNA which you order will work in the first time itself... You must keep some backup money or plans...
You have to separate specifically the general and specific objectives to long and short time; implications, obstacles and limitations.
OK, fine.. So you want to go in a completely different direction by creating alternative life.. Good.. All the best for this project.
Well, if you want to go to the extremes then are you limiting yourself to already established phenomena such as DNA, membranes, etc. You may want to adopt a completely new and novel ideas which might be simpler than DNA and other stuff of the normal cell.
So, keep all the options open. Research the literature well and create Artificial Life...
Questions
- - Dilettante: "Where is a place of synthetic biology here - good old gene engineering > bio-nanotechnology > orthogonal life > artificial life > artificial intelligence?"
- Expert: "Hmmm..." - - Professor: "What is new in Synthetic Biology?"
- Student: "Wiki!!!" - People on a street:
- "What is a synthetic biology - science or technology?"
- "Expedition." - - Shrödinger: “What is life?”
- Alice: “It’s a play where rules are constantly changed.”
Hey Mutant, have a look!
The easy and serious way
- George Church CONSTRUCTIVE BIOLOGY - a nice paper about the challenges of modern biology
- The webcast from SB2.0
These people do great things
Albert Libchaber [2]
Carlos Bustamante's lab [3]
David Deamer [4]
Eric Kool’s group [5]
Erik Winfree [6]
Fred Menger’s group [7]
Jack Szostak’s lab [8]
Norman Packard’s Protolife [9]
Pier Luigi Luisi’s group [10]
Radhika Nagpal [11]
Steven Benner’s group [12]
Local
An Analysis of Synthetic Biology Research in Europe and North America [13] DNA synthesis: ATG-Biosynthetics [14]
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