Generation Counter

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Back to [[ETH Zurich]] main page.
Back to [[ETH Zurich]] main page.
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  ''''This page is no longer updated. For the last details about the generation counter, have a look at [[Oscillator_based]]''''
=Intro=
=Intro=
Cell generation counter.  
Cell generation counter.  
=Principle=
=Principle=
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A counter somehow triggered by cell division that allows the experimentator to trace down the number of generation.
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A counter somehow triggered by cell division that allows the experimentator to track the number of generations.
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=The telomere approach=
=The telomere approach=
In somatic mamalian cells, the telomeres cannot be copied in their full length by the polymerases, because they copy in one direction and so they would need to start *after* the end to copy the ends. That is somewhat the worse explanation you will ever read about this phenomenon, so googling a bit about "telomere" and say, "aging", won't harm you at this point.
In somatic mamalian cells, the telomeres cannot be copied in their full length by the polymerases, because they copy in one direction and so they would need to start *after* the end to copy the ends. That is somewhat the worse explanation you will ever read about this phenomenon, so googling a bit about "telomere" and say, "aging", won't harm you at this point.
Is there a way to use this trick in bacteria? The problem is far from trivial since  
Is there a way to use this trick in bacteria? The problem is far from trivial since  
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# bacteria usually digest linear DNA
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# bacteria usually digest linear DNA and so it won't last for long
# it is difficult to insure that that DNA gets only replicated once per generation (as plasmid tend to have asynchronous replication cycles?!?)
# it is difficult to insure that that DNA gets only replicated once per generation (as plasmid tend to have asynchronous replication cycles?!?)
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Maybe the idea should be implemented in yeast?
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# the determination of the actual number of generations would probably be statistical rather than exact, by filtering "telomeres" of different lengths (since depending on replication and shortening the longer strands will still be present to some extent), e.g. by electrophoresis, and determining the "telomeres" with the shortest length.
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=Discussion=
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Comments:
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- Urs: In the crash course Sven told us, that E coli can share there proteins. Do they do this quite often and could this affect the result?
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=Discussion=
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>> for comments, questions and temporary remarks go to the [[Talk:Generation_Counter]]
Back to [[ETH Zurich]] main page.
Back to [[ETH Zurich]] main page.

Latest revision as of 20:57, 9 August 2005

Back to ETH Zurich main page.

 'This page is no longer updated. For the last details about the generation counter, have a look at Oscillator_based'

Contents

Intro

Cell generation counter.

Principle

A counter somehow triggered by cell division that allows the experimentator to track the number of generations.

The telomere approach

In somatic mamalian cells, the telomeres cannot be copied in their full length by the polymerases, because they copy in one direction and so they would need to start *after* the end to copy the ends. That is somewhat the worse explanation you will ever read about this phenomenon, so googling a bit about "telomere" and say, "aging", won't harm you at this point. Is there a way to use this trick in bacteria? The problem is far from trivial since

  1. bacteria usually digest linear DNA and so it won't last for long
  2. it is difficult to insure that that DNA gets only replicated once per generation (as plasmid tend to have asynchronous replication cycles?!?)
  3. the determination of the actual number of generations would probably be statistical rather than exact, by filtering "telomeres" of different lengths (since depending on replication and shortening the longer strands will still be present to some extent), e.g. by electrophoresis, and determining the "telomeres" with the shortest length.

Discussion

>> for comments, questions and temporary remarks go to the Talk:Generation_Counter

Back to ETH Zurich main page.

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