Lab Notebook
From 2006.igem.org
May 15: Transformed Top10F' bacteria with the pGFP vector containing the Luciferase gene (hopefully the ligation worked this time).17:22, 15 May 2006 (EDT)
May 16: Transformation of Top10F did not work. The cause was an error in the extraction of the DNA from the the agarose gel. Completed a new agarose gel extraction of pGFP plasmid DNA, as cut by EcoRI and HindIII. Prepared a new seed of Top10F bacteria transformed with luciferase. Prepared a midiprep seed of EcoMscL.
May 17: The control of the miniprep of luciferase was murky. This could have been due to contaminants in the LB, water, ampicillin, or pipet tips. A miniprep of both luciferase and EcoMscL was conducted. A new seed of luciferase was prepared. The miniprep of luciferase was digested with EcoRI and HindIII. The miniprep of EcoMscL was digested with XmnI. A gel with the digests was run but the EcoMscL showed no bands and the luciferase only had faint bands.
May 18: The controls of the seed of luciferase were clear. A miniprep of the new luciferase seed and the old EcoMscL seed was conducted. The same restriction enzymes were used to digest the plasmids. A gel was run with the digests.
