McGill University 2006

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
 
(37 intermediate revisions not shown)
Line 7: Line 7:
Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!
Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!
-
[[Image:Clip_image002.jpg]][[Image:Dancing e copyzz.jpg]]
+
[[Image:Clip_image002.jpg]][[Image:Good_2.jpg]]
-
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"
+
<!--{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"
|-valign="top"
|-valign="top"
|width=179.25px style="padding: 5px; background-color: #fffaf0; border: 2px solid #20b2aa;" |
|width=179.25px style="padding: 5px; background-color: #fffaf0; border: 2px solid #20b2aa;" |
-
<center><h2>Projects</h2></center>
+
EDITED BY ASHWIN. If you want to change it, feel free :)-->
-
* '''Team 1: Split YFP'''
+
-
The idea behind the project is fluorescence complementation, which involves the joining of two leucine zipper proteins Jun and Fos each fused to a half terminus of YFP.  
+
-
Subsequently, both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. The project involved performing a PCR reaction to produce two inserts, the
+
-
N-terminus and the C-terminus of the YFP, and then ligating these inserts into 2 vectors, containing the
+
-
Jun-beta and the Fos-beta respectively.
+
-
We transformed two cell populations, one expressing Jun-beta-YFPN and the other expressing Fos-beta-YFPC.These two cell populations were combined and the two vectors were then expressed, ideally resulting in the fusion of the Jun and Fos leucine zipper proteins on the cell membrane when the cells are in close contact. This would result in the binding of the two halves of the YFP protein resulting in flourescence.
+
 +
<center><h2><font face="broadway,verdana">Projects</font></h2></center>
 +
<table cellpadding="5" border="0"><tr>
 +
<td valign="top" width="50%">
 +
<h3><ul><li>'''Team 1: Split YFP'''</ul></h3>
 +
This project on fluorescence complementation involves the joining of two proteins, Jun and Fos, each fused to a half terminus of YFP. Both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Then, two cell populations - one expressing Jun-beta-YFPN and the other Fos-beta-YFPC - were combined, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. The two halves of the YFP protein would bind as well, giving rise to fluorescence.
-
'''* Team 2: Repressilator'''
+
[[Background|Background]]
-
We are working on making a biological oscillator.  This system will consist of the Ellowitz repressilator and quorum sensing.
+
[[Methods and Materials|Methods and Materials]]
-
[[Oscillator Example|Oscillator Example]]
+
[[Results|Results]]
 +
 
 +
[[Future Prospects|Conclusions and Future Work]]
 +
 
 +
</td>
 +
 
 +
<td valign="top" width="50%">
 +
<h3><ul><li>''' Team 2: Repressilator'''</ul></h3>
 +
 
 +
Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations.
[[Theory Behind the Oscillator|Theory Behind the Oscillator]]
[[Theory Behind the Oscillator|Theory Behind the Oscillator]]
 +
[[McGill_Repressilator_M_and_M|Methods and Materials]]
-
<center><h2>Lab Procedures</h2></center>
+
[[McGill_Repressilator_Results|Results]]
 +
</td>
 +
 
 +
</tr></table>
 +
 
 +
<center><h2><font face="broadway,verdana">Lab Procedures</font></h2></center>
 +
<table border="0"><tr><td valign="top" width="85%">
* [[Protocols]]
* [[Protocols]]
* [[Lab Notebook]]
* [[Lab Notebook]]
-
 
+
</td>
 +
<td>[[Image:Test_tubes.jpg]]</td></tr></table>
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"
|-valign="top"
|-valign="top"
-
<center><h2>Club</h2></center>
+
<center><h2><font face="broadway,verdana">Club</font></h2></center>
* [[News]]
* [[News]]
* [[Team Members]]
* [[Team Members]]
Line 48: Line 64:
* [[Journal Club Papers]]
* [[Journal Club Papers]]
-
 
+
<center><h2><font face="broadway,verdana">Just for Fun</font></h2></center>
-
<center><h2>Just for Fun</h2></center>
+
* [[iGEM Party Pictures]]
* [[iGEM Party Pictures]]
* [[ahessel:montreal|Ambassador Visit]] [http://www.flickr.com/search/?q=igem+montreal&m=text Photos]
* [[ahessel:montreal|Ambassador Visit]] [http://www.flickr.com/search/?q=igem+montreal&m=text Photos]

Latest revision as of 20:19, 31 October 2006

Banner.gif


Welcome to the McGill Wiki! Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!

Clip image002.jpgGood 2.jpg



Projects

  • Team 1: Split YFP

This project on fluorescence complementation involves the joining of two proteins, Jun and Fos, each fused to a half terminus of YFP. Both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Then, two cell populations - one expressing Jun-beta-YFPN and the other Fos-beta-YFPC - were combined, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. The two halves of the YFP protein would bind as well, giving rise to fluorescence.

Background

Methods and Materials

Results

Conclusions and Future Work

  • Team 2: Repressilator

Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations.

Theory Behind the Oscillator

Methods and Materials

Results

Lab Procedures

Test tubes.jpg

Club

Just for Fun

Poutine.gif


Personal tools
Past/present/future years