McGill University 2006
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| + | <small>See [http://www.flickr.com/search/?q=igem+montreal&m=text photos] from the [[ahessel:montreal|Ambassador visit]] on May 31, 2006.</small> | ||
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Revision as of 22:29, 20 June 2006
Contents |
Name: Fousion
See photos from the Ambassador visit on May 31, 2006.
News
- 2006-06-15 1 month anniversary of attempting to ligate pGFP & Luciferase :) :)
- 2006-06-02 Put up some profile pictures
- 2006-05-31 iGEM Party Pictures!
- 2006-05-15 Lab Notebook page created
- 2006-04-14 Discussion page created
- 2006-03-16 First kick-off meeting and team selection
Organisation
People
Students
Supervisors
| Jay Nadeau |
Timeline
Tasks
Modelling people: check out these bricks: BBa_I13920, BBa_I13921, BBa_I13922. What happens if you plug these into your computer simulation?
Also try this: BBa_J11002 and BBa_J06913. What happens if you substitute an AAV-degradation-tagged GFP?
Test EcoMscL to see if it does anything cool How many vials of competent Mscl(-) E. coli do we have? *make more* Do we have a luciferase reporter? What is its selection marker Amplify the Eco MscL plasmid
Useful papers
A synthetic gene–metabolic oscillator Eileen Fung1,2, Wilson W. Wong1, Jason K. Suen1, Thomas Bulter1, Sun-gu Lee1 & James C. Liao1,2 NATURE | VOL 435 | 5 MAY 2005 http://www.nature.com/nature/journal/v435/n7038/abs/nature03508.html
Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):679-84. Synchronizing genetic relaxation oscillators by intercell signaling. McMillen D, Kopell N, Hasty J, Collins JJ. http://www.pnas.org/cgi/content/full/99/2/679
Cell Mol Life Sci. 2006 May
The design of intracellular oscillators that interact with metabolism.
Wong WW, Liao JC.
Parts shopping list
=Introduction= Our brainstorming meeting on 3-16 gave us several preliminary ideas which we will explore via modeling and research Things I learned at the teach the teachers meeting
Standard plasmids: pSB1AK3 ("ampicillin kanamycin")
pSB1AT3 ("amplicillin tetracycline)
pSB1AC3 ("ampicillin chloramphenicol)
all of these are high copy number, if inappropriate, also provided are inducible plasmids:
pSB2K3 (IPTG inducible)
the "3" refers to the MCS and the transcriptional terminator We will be provided with a plate with dried DNA samples, we can amplify the ones that we want There are standard cloning protocols that use specific sites; products are checked by sequencing with promoter sequences VF ("verification forward") and VR ("verification reverse")
They often use 3-way ligations selected with 2 antibiotics to create composites You can put > 1 plasmid in a cell; chose according to copy number desired (eg, you might want GFP at high copy and AraC at low...)
EVERYONE should have a BioBrick account with their real name
go here:
http://partsregistry.org/Help:Create_a_Registry_Account
It is important to document parts every time we create them
They should have the "BioBrick ends" what does this mean?
We will get 2 384-well plates with the DNA; pierce the foil, dissolve in 30-50 microliters, and transform it's colour-coded with food coloring: KANAMYCIN = red; TET = yellow; CHLOR = green; AMP = orange the key to what's in each well will be provided on the registry fill out address on Group pages next week! when we send parts, they should be sent as stabs labeled w/ part name, plasmid, cell (on Help page) (or foreign schools can send dried DNA) as soon as a part is Available, it will be sent to EVERYONE, that way they don't worry about shopping lists The letters on the plates are very small, so watch out
